Extended Data Table 1: Overview of Ca2+ binding rsGCaMP variants generated and proteins considered in this work. | Nature Biotechnology

Extended Data Table 1: Overview of Ca2+ binding rsGCaMP variants generated and proteins considered in this work.

From: Genetically encoded photo-switchable molecular sensors for optoacoustic and super-resolution imaging

Extended Data Table 1

a, Overview of the mutations introduced into GCaMP5G to generate the variants presented in this work. rsEGFP2 numbering is additionally indicated for comparison. b, Photophysical and Ca2+ binding data for variants from (a). All data was recorded as experimental triplicates (n=3) with the mean and standard deviation given. Photo-switching was achieved using a 405 / 12.5 nm LED and a 490 / 26 nm LED. Light was delivered with a 5 mm core diameter liquid light guide to the 50 µl cuvette, illuminating the whole volume from the top with 1528 mW/cm2 and 279 mW/cm2 at 405 and 488 nm, respectively28,29,69.

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