Extended Data Fig. 4: 2H11 CLAMP enables the discovery of weak affinity SWII ligands.
From: Conformation-locking antibodies for the discovery and characterization of KRAS inhibitors
(a) 2H11 CLAMP enhances the binding of SWII pocket ligands to KRASWT and KRASG12C. KRAS was immobilized on an SPR chip and small molecule was injected at concentrations ranging from 1 to 50 μM in the absence or presence of 2H11 CLAMP. (b) Unbiased omit map, 2Fo-Fc contoured at 1σ of GNE-2897 bound in the SWII region of KRASG12C + 2H11 CLAMP Fab.
