Extended Data Fig. 3: Biophysical and structural analysis of 2H11 CLAMP.
From: Conformation-locking antibodies for the discovery and characterization of KRAS inhibitors
(a) Structural alignment of 2H11 CLAMP bound to KRASG12C-GDP and DCAI ligand bound to KRAS (PDB 4DST). KRASG12C is depicted as white ribbons with SWII highlighted in blue. 2H11 CLAMP heavy chain is colored in green, and Trp99 rendered in sticks. DCAI bound KRASG12D is colored in red, with DCAI shown rendered in sticks. DCAI and Trp99 bind to the same site on KRAS. (b) Structural alignment of KRASG12C-GDP/GNE-1952 with and without 2H11 CLAMP bound. KRASG12C/GNE-1952 without 2H11 CLAMP is colored in cyan. KRASG12C/GNE-1952 with 2H11 CLAMP is colored in orange, compound is colored in yellow, and 2H11 CLAMP heavy chain is colored in green. (c) Structural comparison between iDab (orange, PDB 2UZI) and 2H11 CLAMP (green) in SWI (teal) binding suggest less sensitivity to different nucleotide states for 2H11 CLAMP.
