Extended Data Fig. 5: Comparison of twinPE and PE3 for Bxb1 attB insertion at CCR5. | Nature Biotechnology

Extended Data Fig. 5: Comparison of twinPE and PE3 for Bxb1 attB insertion at CCR5.

From: Programmable deletion, replacement, integration and inversion of large DNA sequences with twin prime editing

Extended Data Fig. 5

(a) Replacement of endogenous sequence within CCR5 region 1 with the Bxb1 attB site using twinPE or PE3. For PE3 editing systems, pegRNA RT templates were designed to encode the Bxb1 attB sequence and one of three different target-site homology sequence lengths. For PE3 edits, each pegRNA was tested with three nicking sgRNAs. (b) Replacement of endogenous sequence within CCR5 region 2 with the Bxb1 attB sequence using twinPE or PE3. As in (a), PE3 edits were tested with pegRNAs containing RT templates that were designed to encode the Bxb1 attB sequence and one of three different target-site homology sequence lengths and tested with three nicking sgRNAs. Values and error bars in (a) and TwinPE edits, PE3 edits of CCR5_D2_23, CCR5 D2_28 with nicking guide RNA C1 and C1.5 in (b) reflect the mean and s.d. of three independent biological replicates. Values of CCR5 D2_28 with nicking guide RNA C4 and CCR5 D2_34 in (b) reflect the mean of two independent biological replicates.

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