Extended Data Fig. 5: (matched to Fig. 6).
a. Schematic of experimental design of dexamethasone treatment: SARS-CoV-2 infected MISTRG6-hACE2 mice were either treated with dexamethasone on days 7, 8, 9 post-infection or left untreated. Mice were analyzed either at 14 dpi or 28 dpi. Mice were treated intraperitoneally (i.p.) with dexamethasone at 10mg/kg dose. b. Representative flow cytometry plots of Ly6G expressing SSChi cells (mouse neutrophils) within the mouse immune cell population (mouse CD45+) in the BAL of dexamethasone treated or control untreated mice. Representative of 4 biologically independent mice examined over at least 2 independent experiments. c. HLA-DR expression on lung T cells 28 dpi in dexamethasone treated or control mice. Representative of 3 biologically independent mice examined over at least 2 independent experiments. d. Representative flow cytometry plots of Surface IgG and CD19 expression on human immune cells gated on hCD45+ cells in lungs of untreated or dexamethasone treated mice at 28 dpi. N=3 of biologically independent mice examined over at least 2 independent experiments. e. Frequencies of IgM+ and IgG+ B cells in lungs of untreated or dexamethasone treated mice at 28 dpi. Mice were treated with dexamethasone on days 7,8,9 post-infection. N=3 biologically independent mice examined over at least 2 independent experiments. Unpaired t-test, two-tailed. f. Viral RNA in the lung homogenates of dexamethasone treated or control untreated mice at 28dpi. Mann-Whitney, two-tailed test. N=5 biologically independent mice examined over 3 independent experiments. g. Schematic of experimental design of SARS-CoV-2 infected MISTRG6-hACE2 mice either treated with dexamethasone on days 3, 4, 5 dpi or left untreated. h. CD206 and CD68 expression in lung human immune cells in mice treated with dexamethasone or left untreated at 7dpi. CD206hi+CD68+ cells are alveolar macrophages. Treated N=4, untreated N=6 biologically independent mice examined over 2 independent experiments. Alveolar macrophages are marked by high CD206 expression. Inflammatory macrophages are enriched in the CD206 negative population which also express CD86 (not shown).
