Extended Data Fig. 8: Comparison of the prime editing efficiency induced by PPE or ePPE with NGG-pegRNA, CCN-pegRNA and dual-pegRNA strategies.
From: An engineered prime editor with enhanced editing efficiency in plants
(a) Frequencies of prime editing induced by PPE and ePPE at six rice target sites using NGG-pegRNA, CCN-pegRNA and dual-pegRNA strategies. The edits were referred to the base on the DNA forward strand. (b) Overall editing frequencies induced by PPE and ePPE containing NGG-pegRNA, CCN-pegRNA and dual-pegRNA. The average editing frequencies using ePPE-dual-pegRNA for each target were normalized to 1, and the frequencies using others for each target were adjusted accordingly. (c) Product purity in prime editing by PPE and ePPE using NGG-pegRNA, CCN-pegRNA and dual-pegRNA strategies. Data and error bars reflect the mean and standard deviation of three independent biological replicates. Frequencies (means ± s.e.m.) were calculated from three independent experiments (n = 3). P values were obtained using two-tailed Student’s t-tests. **P < 0.01, ****P < 0.0001.
