Extended Data Fig. 6: Prime editing using mutant PE2 and sPE components.
From: A split prime editor with untethered reverse transcriptase and circular RNA template
Representative Sanger sequencing traces from prime editing experiments using mutant PE2 and sPE components. HEK293T cells were transfected with indicated plasmids, along with others encoding pegRNA and nicking sgRNA. Prime editing introduces a 3-nt substitution at the FANCF locus (+2 C to T and +4-5 TG to AC). The experiment was repeated two times. Sanger sequencing traces were analyzed by EditR.
