Extended Data Fig. 10: In vitro transcribed mRNA and purified RT protein used in the nucleofections, and FACS gating strategy. | Nature Biotechnology

Extended Data Fig. 10: In vitro transcribed mRNA and purified RT protein used in the nucleofections, and FACS gating strategy.

From: A split prime editor with untethered reverse transcriptase and circular RNA template

Extended Data Fig. 10

a, Denaturing agarose gel analysis of the mRNAs produced in-house. The coding sequences of nCas9, MMLV-RT or PE2 were flanked by a capped 5’ UTR and a 3’ UTR, followed by a 110-nt poly(A) tract. b, SDS-PAGE analysis of the purified MMLV-RT protein. C, FACS gating examples for reporter cells.

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