Extended Data Fig. 1: MS2-PE2 and SunTag-PE2 design.
From: A split prime editor with untethered reverse transcriptase and circular RNA template
a, Schematic overview of MS2-PE2. The MS2 coat protein (MCP) was fused to the N terminus of M-MLV reverse transcriptase to enable recruitment by the MS2-pegRNAs. b, Sizes of Cas9 nickase and MCP-RT ORFs. c, Engineered MS2-pegRNAs with MS2 sequences appended into distinct sgRNA stem-loops, or onto the 3′ terminus. d, Schematic overview of SunTag-PE2. e, Schematics of scFv-RT and GCN4-Cas9 nickase. The scFv was fused to the N terminus of M-MLV RT (top). The 10xGCN4 epitope was fused to either the N terminus (SunTag-PE) or the C terminus of SpyCas9H840A (PE-SunTag).
