Extended Data Fig. 3: SunTag-PE3 and MS2-PE3 tested in reporter lines. | Nature Biotechnology

Extended Data Fig. 3: SunTag-PE3 and MS2-PE3 tested in reporter lines.

From: A split prime editor with untethered reverse transcriptase and circular RNA template

Extended Data Fig. 3

a, A diagram of the GFP reporter line that is activated by precise insertion of 18 bp (in place of a 39-bp non-functional sequence). Indels (+1) can restore mCherry expression. b, A diagram of the GFP reporter line that is activated by deletion of 47 bp; indels (+1) can restore mCherry expression. c, MS2-PE3 was tested in the GFP reporter line shown in panel a (n = 3). d, SunTag-PE3 was tested in the GFP reporter line shown in a (n = 3). e, MS2-PE3 was tested in the GFP reporter line shown in b (n = 3). Data and error bars indicate the mean and standard deviation of three independent biological replicates.

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