Extended Data Fig. 10: Notable gene groups associated with outgrowing/likely outgrowing and depleting/likely depleting sgRNAs and variants.
From: High-throughput functional evaluation of human cancer-associated mutations using base editors
a, (Left panel) The fraction of functionally classified sgRNAs (top) targeting cancer gene census (CGC)5 genes and primary protein variants (bottom) encoded by CGC genes in the outgrowing and likely outgrowing groups. Results from all libraries except library eC were combined. P-values from two-sided Fisher’s exact test are shown. The number of sgRNAs or variants either targeting or encoded by CGC genes among all sgRNAs or variants in each group are shown on the x-axes. (Right panel) Detailed distribution of sgRNAs predicted to introduce mutations in CGC genes (top) and variants generated in CGC genes (bottom). The number of sgRNAs or variants corresponding to each gene is specified in parentheses. b, The fraction of functionally classified sgRNAs (left) targeting Depmap common essential genes (CEGs) and protein variants (right) encoded by CEGs in the depleting and likely depleting groups. Results from all libraries except library eC were combined. P-values from two-sided Fisher’s exact test are shown. The numbers of sgRNAs or variants either targeting or encoded by CEG genes among all sgRNAs or variants in each group are shown on the x-axes.
