Extended Data Fig. 5: Volumetric 3D-SIM imaging of S protein in a SARS-CoV-2-infected VeroE6/TMPRSS2 cell.
From: A highly photostable and bright green fluorescent protein
The Nb(S1) = = StayGold fluorescence (green) was 3D-SIM reconstructed, whereas DAPI fluorescence (blue) was not. Images were acquired from z = 0 to z = 4.56 μm in steps of 0.12 μm upward (in the direction of a yellow arrow). A volume of 9.78 × 9.78 × 4.56 μm3 is 3D rendered. Two single-layer (xy) images at z = 1.44 and 3.24 μm are shown. Scale bar, 2 μm. The z position of the xy image of Fig. 5g is indicated. The volumetric imaging experiment in z steps of 0.12 μm was repeated 4 times with 39–79 slices. In each case, the fluorescence in the last frame (slice) was considerably strong for reliable super-resolution imaging.
