Fig. 2: Comparison of photobleaching curves.
From: A highly photostable and bright green fluorescent protein
a–c, Plotted as intensity versus normalized total exposure time, with an initial emission rate of 1,000 photons/s/molecule. See Supplementary Table 1. a, StayGold variants were photobleached under continuous WF illumination (5.6 W cm−2). b, Various colored FPs were photobleached under continuous WF illumination (3.4–5.6 W cm−2). Inset: A photoactivation component was noted for mOrange2 and mCardinal. All curves shown in Fig. 1h (top) are incorporated here. c, Multimeric FPs were photobleached under continuous WF illumination (3.4–5.6 W cm−2). The curve of StayGold shown in Fig. 1h is incorporated in a and b.
