Extended Data Fig. 3: Visualizing mitochondrial fission and fusion by StayGold SIM technology.
From: A highly photostable and bright green fluorescent protein
upper, HeLa cells stably expressing mt-(n1)StayGold were photographed by wide-field (WF) microscopy. The uniformity of fluorescence intensity among observed cells of this cell line is evident. lower, HeLa cells stably expressing mt-(n1)StayGold were imaged by 3D-SIM continuously with an irradiance value of 3.9 W/cm2. Shown are two representative experimental results. In one experiment, which spanned 1 min 24.133 sec, Cell #22 was imaged at 4.4 Hz. In the other experiment, which spanned 1 min 27.014 sec, Cell #24 was imaged at 5.3 Hz. SIM images in boxed regions (ROIs) at the indicated times (min: sec) are presented to show fission (yellow arrowhead) and fusion (red arrowhead) events detected during the observation time periods. Each event is highlighted by a triad of images. Scale bars, 5 μm. Similar results were obtained from 2 other independent cultured cell samples.
