Extended Data Fig. 5: Monoclonal antibodies tested for lucCageRBD, binding, and neutralization.
a–f, Different concentrations of either CV30 (a-c) or B38 (e-g) mAbs with 5 nM RBD WT, Alpha, and Beta were tested in the lucCageRBD assay (a, d), BLI for binding to RBD strains (b, e), and spike VoC-presenting pseudovirus infection (VSV-based for c, HIV-based for f). Comparison of lucCageRBD EC50 and neutralization IC50 plotted linear-linear (left) or log-log (right) (g), binding affinity (kd) and neutralization IC50 (h), and lucCageRBD EC50 and kd (i). All lucCageRBD and BLI experiments were performed in triplicate, neutralization experiments were performed in at least duplicate and data are mean ± s.e.m.
