Extended Data Fig. 6: Detection of neutralizing antibodies in vaccinated serum.
a,b, Serum (10%) from vaccinated mice were collected and tested for pseudovirus infection (a), in ELISA (log10 reciprocal EC50 titer) (b), and in the lucCageRBD assay (a, b) (n=12 serum samples). (Inset) Mouse serum samples pre-immunization, post-prime dosing (week 3), and post-boost dosing (week 5) were collected and tested in the lucCageRBD assay. c-d, Serum (10%) from vaccinated patients were tested for pseudovirus infection (c), in ELISA (log10 reciprocal EC50 titer) (d), and in the lucCageRBD assay (c, d) using RBD WT, Alpha, and Beta (n=12 serum samples). e-f, Serum collected from patients pre and post vaccination were tested in the EuroImmun ELISA for anti-spike antibodies. Patient’s anti-spike antibody levels (measured by Z-score) were measured several days after vaccination. Z-score is plotted either in linear (e) or log10 scale (f). All neutralization experiments were performed in at least duplicates, all lucCageRBD and ELISA experiments were performed in triplicate and data are mean ± s.e.m.
