Extended Data Fig. 7: The dose-dependent immune responses elicited by M1-PTD in WT mice and the immune responses elicited by M1-PTD in BALB/c Nude mice.
From: Generation of a live attenuated influenza A vaccine by proteolysis targeting
a, HI antibody responses elicited by indicated dosages of M1-PTD in the sera of WT BALB/c (left) and BALB/c Nude (right) mice at day 21 post-vaccination (n = 5). Data are plotted for individual mice and overlaid with mean ± SD; left graph, one-way ANOVA with Dunnett’s multiple comparisons test; right graph, unpaired two-tailed t-test; n.s., not significant. b, NT antibody responses elicited by indicated dosages of M1-PTD in the sera of WT BALB/c (left) and BALB/c Nude (right) mice at day 21 post-vaccination (n = 5). Data are plotted for individual mice and overlaid with mean ± SD; left graph, one-way ANOVA with Dunnett’s multiple comparisons test; right graph, unpaired two-tailed t-test; n.s., not significant. c, Anti-HA antibody responses elicited by indicated dosages of M1-PTD in the sera of WT BALB/c (left) and BALB/c Nude (right) mice at day 21 post-vaccination (n = 5). Data are plotted for individual mice and overlaid with mean ± SD; left graph, one-way ANOVA with Dunnett’s multiple comparisons test; right graph, unpaired two-tailed t-test; n.s., not significant. d, Anti-NP antibody responses elicited by indicated dosages of M1-PTD in the sera of WT BALB/c (left) and BALB/c Nude (right) mice at day 21 post-vaccination (n = 5). Data are plotted for individual mice and overlaid with mean ± SD; left graph, one-way ANOVA with Dunnett’s multiple comparisons test; right graph, unpaired two-tailed t-test; n.s., not significant. Note that M1-PTD did not induce immune response in T cell-deficient BALB/c Nude mice, indicating that T cell response may be the driver for the immunity elicited by M1-PTD. e, Viral M1 antigen-specific T cell responses elicited by indicated dosages of M1-PTD in the lungs of WT C57BL/6 J mice at day 7 post-vaccination (n = 5), measured by ELISpot assay. Two peptides, M1128-135 MGLIYNRM (left) and M158-66 GILGFVFTL (right), were used as the stimuli. IFNγ-expressing cells per million cells were shown. Data are plotted for individual mice and overlaid with mean ± SD; one-way ANOVA with Dunnett’s multiple comparisons test.
