Extended Data Fig. 8: T cell responses induced by M1-PTD and M1 antigen presentation mediated by PTD.
From: Generation of a live attenuated influenza A vaccine by proteolysis targeting
a, CD8 and CD4 T cell responses elicited by 105 PFU of M1-PTD, CAIV, and M1-KO vaccines in lungs of C57BL/6 J mice at day 7 post-vaccination, measured by flow cytometry. Data are plotted for individual mice and overlaid with mean ± SD; n = 3 mice for Vehicle in left graph, 4 mice for CAIV in left graph, and 5 mice for all other groups; one-way ANOVA with Tukey’s multiple comparisons test. b, Representative flow cytometry histograms are shown for lung samples from mice vaccinated with 105 PFU of M1-PTD. Purple, stained cells; red, unstained cells. c and d, PTD-mediated M1 degradation increases M1 antigen presentation in Raw264.7 cells. Raw264.7 cells were infected with M1-PTD or M1KO virus (MOI = 0.1). 24 hours after infection, M1 antigen presentation on the surface of Raw264.7 cells was detected by an anti-M1 peptide (M1128-135: MGLIYNRM) antibody (c) or an anti-M1 antibody (d) using flow cytometry. Data are presented as mean ± SD; n = 3 biological replicates; unpaired two-tailed t-test.
