Extended Data Fig. 9: Characterization of the broad applicability of PROTAC virus vaccine strategy.
From: Generation of a live attenuated influenza A vaccine by proteolysis targeting
a, Generation and characterization of PROTAC virus M1-PTDPR8 by incorporating PTD to M1 protein of influenza A/Puerto Rico/8/1934 (H1N1) virus (PR8). Characterization of the efficient propagation of M1-PTDPR8 in MDCK-TEVp cells and not in conventional MDCK.2 cells by CPE measured by CellTiter-Glo assay. Data are presented as mean ± SD; n = 3 biological replicates. b, Multicycle replication kinetics curves of M1-PTDPR8 in MDCK-TEVp and conventional MDCK.2 cells. The detection limit is 70 PFU/mL. Data are presented as mean ± SD; n = 3 biological replicates.
