Fig. 3: A mutually dependent CD52 and CD20 antibody combination induces selective depletion of B cells.
From: Logic-gated antibody pairs that selectively act on cells co-expressing two antigens
a–d, Cytotoxicity induced by IgG1-Campath and IgG1-11B8 antibody variants (10 µg ml−1 final concentration) incubated for 18 h in healthy human whole blood, as analyzed by flow cytometry. PBMC viability after 18 h exceeded 90% for all nontreated control samples. a, The fraction (%) of B or T cells remaining within the lymphocyte population (CD66b−) is shown for one representative donor. b,c, Summary of cytotoxicity results relative to a nontreated (no antibody) control sample for five donors containing variable levels of B (b) and T cells (c). d, B cell depletion induced by mutually dependent IgG1-Campath and IgG1-11B8 antibody combinations compared to that of different existing CD20 antibody molecules, shown for three donors relative to a nontreated (no antibody) control sample. e, PBMCs isolated from the blood of six healthy donors were incubated for 20–24 h in the presence of 10 µg ml−1 of each antibody mixture and 20% (V/V) complement-competent NHS. Supernatant cytokine levels were analyzed by MSD QuickPlex Cytokine analysis. Interferon gamma levels shown were measured for six donors, in three individually incubated samples per donor, collected in two experiments. A mixture of anti-CD3 and IL-15, anti-CD3 and anti-CD28 antibodies immobilized on beads, phytohemagglutin (PHA) and lipopolysaccharide (LPS) served as positive control stimuli of different cytokine responses.
