Extended Data Fig. 8: Behavior-guided transcriptomics of TEGs co-cultured with 13T and 10T organoids.
From: Uncovering the mode of action of engineered T cells in patient cancer organoids
(a) Heatmap showing normalized gene expression of behavior-enriched TEG populations co-cultured with 10 T or 13 T organoids, or cultured without PDOs (No target control). Columns represent cells ordered by TEG populations and rows represent the expression of genes. Shown are 534 genes induced upon prolonged organoid engagement (super engagers) in both 10 T and 13 T co- cultures from Fig. 6b. (b) Functional enrichment analysis (conserved biological processes and pathways) of genes induced in both 10T- and 13T-co-cultured super engager TEGs (shown in A). (c) Functional enrichment analysis of genes differentially expressed between 10T- and 13T-co- cultured super engager TEGs. Top differentially regulated biological processes and pathways are shown. (d) IFN-ß concentration measured for the different organoid cultures in Fig. 6e-g.
