Extended Data Fig. 3: Quality metrics for matched 5′ and 3′ PBMC libraries sampling reads to the same number of UMIs.
From: Mostly natural sequencing-by-synthesis for scRNA-seq using Ultima sequencing
(a) Number of cells identified by Cell Ranger only in Ultima, only in Illumina, or both. (b) Distribution of the number of genes per cell. (c) Scatter plots comparing gene expression in Ultima and Illumina sequencing. (d) Scatterplots comparing reads sampled from the same sequencing run. For (c) and (d), one point for each gene as in Fig. 2e. For all 3′ libraries, the last three UMI bases were trimmed for quality reasons.
