Fig. 1: EEL method and 440-gene mouse dataset.
From: Scalable in situ single-cell profiling by electrophoretic capture of mRNA using EEL FISH
a, Schematic illustration of the EEL protocol, including RNA transfer by electrophoresis, capture on the ITO slide, tissue removal and cyclic fluorescent decoding. b, Results of EEL on a sagittal mouse brain section, where every dot is a single molecule of RNA belonging to one of the 30 selected genes to highlight large anatomical structures. The total experiment contained 440 genes. c,d, Zoomed-in results for all genes around the lateral ventricle (c) and cerebellum (d). e–g, High-resolution imaging (<500 nm) yields subcellular resolution (e,f) and cell type marker expression patterns for small anatomical features (g).
