Extended Data Fig. 5: Interactions between endoplasmic reticulum (ER) and peroxisomes (POs) as seen by rDL GI-SIM. | Nature Biotechnology

Extended Data Fig. 5: Interactions between endoplasmic reticulum (ER) and peroxisomes (POs) as seen by rDL GI-SIM.

From: Rationalized deep learning super-resolution microscopy for sustained live imaging of rapid subcellular processes

Extended Data Fig. 5

a, Comparisons of single raw SIM image (left), rDL denoised raw image (middle) and rDL GI-SIM image (right) in the case of two-color live-cell imaging of ER and POs. Scale bar, 2μm. b, Time-lapse two-color images of ER and POs, and the corresponding dynamic characteristics of Mander’s overlap coefficient (MOC) for POs that are stably contacted with ER over time (top and middle rows) or that is freely moving without stable association with ER (bottom row). Scale bar, 0.5 μm. c, Distribution of the ER-PO MOCs’ standard deviation (S.D.) versus their mean values, n = 137. d, Time-lapse images of a tubular ER generation event by hitchhiking on a moving PO. Scale bar, 1 μm. Gamma value, 0.7 for rDL GI-SIM images of ER, and 0.8 for rDL GI-SIM images of POs.

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