Extended Data Fig. 6: Visualizing the behaviors and interactions of NPM1 and RPA49 during cell mitosis via rDL LLS-SIM. | Nature Biotechnology

Extended Data Fig. 6: Visualizing the behaviors and interactions of NPM1 and RPA49 during cell mitosis via rDL LLS-SIM.

From: Rationalized deep learning super-resolution microscopy for sustained live imaging of rapid subcellular processes

Extended Data Fig. 6

a, 3-D rendering of three-color time-lapse images of fibrillar centre (FC, labeled by Halo-RPA49) and granular component (GC, labeled by mEmerald-NPM1), and chromosome (labeled by mCherry-H2B) at different stages of mitosis in a HeLa cell, knocking-in mEmerald-NPM1 and stably expressing mCherry-H2B and Halo-RPA49. b, Time-lapse max intensity projections of NPM1 and RPA49 showing that the dissociation of RNA polymerase I subunits (e.g., RPA49) from the innermost FC preceded the disassembly of the outermost GC during prophase to prometaphase. c, Time-lapse max intensity projections of NPM1 and RPA49 showing that small nucleolar-derived foci (NDFs) of NPM1 coalesced into giant ones (indicated by arrows in the second row), while the innermost RPA49 marked FC foci of large size could be partitioned into several independent small foci during telophase (arrows in the third row). Scale bar, 6 μm (a), 3 μm (b), 2 μm (c).

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