Extended Data Fig. 4: MulTI-Tag across diverse target combinations.
From: Multifactorial profiling of epigenetic landscapes at single-cell resolution using MulTI-Tag
a) Schematic describing single cell MulTI-Tag profiling different combinations of targets in the same combinatorial indexing experiment. One of four targets (PolIIS5P, H3K9me3, H3K4me1, or H3K36me3) was tagmented in sequence with H3K27me3 in bulk, then arrayed in a 96 well plate as displayed for i7 tagmentation (Methods). b) Violin plots describing distributions of unique reads per cell in K562 cells (left), H1 cells (center), or the K562-H1 cell mixed population (right) for the experiments described in a). Median values for H3K27me3 unique reads (red), PolIIS5P unique reads (blue), H3K9me3 unique reads (magenta), H3K4me1 unique reads (orange), or H3K36me3 unique reads (teal) are displayed at the top of each violin. Number of cells described for each cell type-target combination is displayed at the bottom of each violin. c) Connected UMAP plots for single cell MulTI-Tag data from experiments described in a). Projections based on H3K27me3 (center), PolIIS5P (top left), H3K9me3 (bottom left), H3K4me1 (top right), or H3K36me3 (bottom right) are shown. Total cells represented and normalized mutual information (NMI) of cell type cluster accuracy are denoted for each projection. Lines are connected between points that represent the same single cell in different projections.
