Extended Data Fig. 5: Effect of nicking guides on insertion of diverse cargos. | Nature Biotechnology

Extended Data Fig. 5: Effect of nicking guides on insertion of diverse cargos.

From: Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using CRISPR-directed integrases

Extended Data Fig. 5

a) PASTE insertion efficiency at ACTB and LMNB1 loci with two different nicking guide designs. b) Attachment site insertion at the SERPINA1 locus with a panel of different nicking guides at varying distances. c) Effect of nicking guides on PASTE integration efficiency at the LMNB1 locus with two different atgRNA designs. d) PASTE integration efficiency at ACTB and LMNB1 with target and non-targeting spacers and matched atgRNAs with and without BxbINT expression. e) Integration of a panel of different gene cargo at LMNB1 locus via PASTE. Data are mean (n = 3) ± s.e.m.

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