Fig. 5: Magnify–SOFI visualizes ultrafine structures of cellular components.
From: Magnify is a universal molecular anchoring strategy for expansion microscopy
a, Comparison between Magnify and Magnify–SOFI. Top, Cross-section of a basal body in human bronchial basal stem-cell-derived lung organoid processed with Magnify (left) and Magnify–SOFI (right). Bottom left, radial intensity profiles of basal bodies indicated by yellow and green circles. Bottom right, histogram of microtubule bundle peak-to-peak distances. a.u., arbitrary units. b, Electron micrograph of cilia in human stem-cell-derived lung organoid; inset, zoom in view (red box). c, Confocal image of cilia from the same type of tissue as b, expanded by Magnify–SOFI and stained with Alexa Fluor 488-conjugated NHS ester. d, 3D reconstruction of cilia in c. e, Electron micrograph of mitochondria in the same organoid as in b. f, Confocal image of mitochondria from the same expanded organoid as in e. g, Orthogonal view of a mitochondria indicated by the red box in e. EF = 10× h, Maximum intensity projection of a Magnify–SOFI image stack of ependymal cilia and basal bodies from the ependymal cell lining in the adult mouse brain. i, 3D reconstruction of h. j,k, Zoomed in images of individual ependymal cilia in 3D as indicated by the dashed red boxes in i. Yellow arrows indicate distal appendages. EF = 10.5×. Scale bars, a, 50 nm; b,c, 800 nm, Inset, 200 nm; d, x,y, 1 µm, z, 410 nm; e,f, 800 nm, Inset, 200 nm; g, 200 nm; h,i, 500 nm; j,k, 250 nm; scale bars are all in biological scale.
