Extended Data Fig. 9: HIO microenvironment orchestrates GALT formation independently of the timing of hematopoietic reconstitution.
From: In vivo development of immune tissue in human intestinal organoids transplanted into humanized mice
10 weeks after UCB engraftment, HIOs were transplanted and grown for 12 weeks. a. Transplanted HIO at 12 weeks with mouse kidney seen underneath from control or humanized mice. b. Graph represents the length of HIOs from control (n = 5) (black dot) or humanized (n = 4) (green square) group. Mean± standard deviation (SD). Mann-Whitney tests (two-sided); p = 0.1905. c. Graph represents immunophenotyping of peripheral blood from humanized mice at 12 weeks post HIO transplantation (or 22 weeks post UCB engraftment) (n = 4). Mean± standard deviation (SD). d-e.Formalin-fixed paraffin-embedded (FFPE) sections of transplanted HIO at 12 weeks H&E-stained (d) or stained by IHC with anti-human CD45 (e) and anti-human CD3 (brown) and CD20 (red) antibodies (f). Scale bar represents 100 μm.
