Fig. 4: Lymphoid-like structures in transplanted HIOs correlate to lymphoid development in fetal gut.
From: In vivo development of immune tissue in human intestinal organoids transplanted into humanized mice
a, Prevalence of immune developmental features observed in HIO. Representative of two independent experiments21,22. b–f, Images illustrating features observed in HIO that resemble immune cell development described in fetal gut. b–d, HIO sections stained with anti-human CD3 (top) to demonstrate the presence of T cells, anti-human CD4/CD8 (middle) to distinguish T helper cells versus T cytotoxic cells and anti-human CD20 (bottom) to highlight the presence of B cells. e, Hematoxylin and eosin (H&E) staining indicating the presence of neutrophils (black arrowheads) observed in late developing HIOs only. f, H&E staining highlight the presence of plasma cells (black arrowheads, top) then confirmed with anti-human MUM1 IHC staining (bottom). Scale bars, 50 μm. Representative of two independent experiments.
