Extended Data Fig. 1: Validation of the humanized mouse model.
From: In vivo development of immune tissue in human intestinal organoids transplanted into humanized mice
a. Experimental workflow. Human intestinal organoids (HIOs) generated in vitro were then transplanted in humanized or control mice. At 12, 16 and 20 weeks post transplantation, HIOs and tissues of interest were collected for analysis. b-d. Flow cytometry analysis of peripheral blood from humanized mice. Contour plots represent the gating strategy of human immune cell lineages in humanized mice post HIO transplantation (b). Graph represents percentage of total hCD45+ cells (c) or, as indicated, immune cell subsets (d) in peripheral blood of humanized mice at 12 (n = 7), 16 (n = 9) and 20 (n = 3) weeks post HIO transplantation. Mean± standard deviation (SD). e. Graph represents relationship between length of HIO and percentage of blood hCD45 at different time points. Spearman correlation coefficient r = 0.1881 with P = 0.4405 (two-sided). (12 weeks n = 7; 16 weeks n = 9 and 20 weeks n = 3 mice) f. Humanized mouse small intestine at indicated time, were stained with anti-human CD45 by immunohistochemistry (IHC) and couterstained with hematoxylin. Scale bar represents 50 µm.
