Extended Data Fig. 7: Recording of sRNAs OmrB and PinT in Salmonella colonies under different growth conditions.
From: RNA recording in single bacterial cells using reprogrammed tracrRNAs
(a) Experimental setup for infection conditions. (b) Recording of OmrB and PinT in individual colonies following the setup in a. The same values were used in Fig. 5f. (c) Co-recording of OmrB and PinT in individual colonies following the setup in a. Values are plotted for two different positions within the pinT DNA target. The dots comprise measurements from all three biological replicates shown in b. The same values were used in Fig. 5g. (d) Experimental setup for in vitro growth conditions in non-inducing medium (LB) or inducing medium (SPI-2). (e) Recording of OmrB and PinT in individual colonies following the setup in d. For all experimental setups, Salmonella was equipped with the same recording machinery as in Extended Data Fig. 6f with a medium-copy recording plasmid. Each dot in b and e represents one sequenced colony, with clusters of dots representing one of three biological replicates starting from separate colonies.
