Fig. 4: CBE-Ts elicit robust C·G to T·A conversions in human cells at levels comparable to or higher than BE4 with a narrower editing window.
From: Improved cytosine base editors generated from TadA variants
a,b, Maximum C·G to T·A and A·T to G·C conversion in HEK293T cells transfected with mRNA encoding core CBE-T variants, plus controls, across eight targeted genomic loci via synthetic gRNAs at saturating (500 ng mRNA) (a) and subsaturating conditions (62.5 ng construct mRNA + 437.5-ng nontranslated carrier mRNA) (b). c, Percent change in C·G to T·A (left) and A·T to G·C (right) editing rates between CBE-T1 variants and ABE8.20 at each target site position (PAM = positions 21–23) across eight genomic sites tested (sites 1 to 8; Supplementary Table 3). d, Median C·G to T·A conversion at each target window position as specified on the x-axis, with position numbering defined as the PAM designated as positions 21–23. Values for color maps were determined from mRNA transfections at saturating conditions. Values and error reflect the mean and s.d. of n = 4 (saturating conditions) or 3 (subsaturating conditions) independent biological replicates performed on different days.
