Fig. 5: Guide-independent off-target evaluation of CABE-T and CBE-Ts.
From: Improved cytosine base editors generated from TadA variants
a, Odds ratio plot for C to T mutations relative to all other mutation types in cells edited with CABE-T3.155, CABE-T2.19, CBE-T1.14, CBE-T1.46 and CBE-T1.52, BE4, YE1, BE4-PpAPO and BE4-PpAPO-H122A compared to untreated clonally expanded cells, with black bars representing the median odds-ratio for that treatment group (P = 0.8359, 0.7473, 0.9476, 0.8089, 0.9751, 7.770 × 10−5, 0.9859, 0.00148 and 0.7473, respectively; one-sided Mann–Whitney U test). All n = 8 biologically independent single-cell expanded cell populations are shown for each condition. b, Odds ratio plot for A to G mutations relative to all other mutation types in cells edited with CABE-T33.155, CABE-T2.19, CBE-T1.14, CBE-T1.46 and CBE-T1.52 and ABE8.20 compared to untreated clonally expanded cells, with black bars representing the median odds-ratio for that treatment group (P = 0.1641, 0.4796, 0.5204, 0.8607, 0.5204 and 0.2527, respectively; one-sided Mann–Whitney U test). All n = 8 biologically independent single-cell expanded cell populations are shown for each condition. c, In vitro kinetics of A-to-I or C-to-U deamination of the same substrate presented as ssDNA to BE4, ABE8.20 and CBE-T1.14 in the absence of gRNA. Pseudo first-order apparent rate constants (kapp) obtained by fitting to a single exponential fit are reported (mean ± s.d., n = 3 independent replicates). See gel source data.
