Extended Data Fig. 4: Expression of CD64 on target cells inhibits anti-B7-H3 antibody-mediated ADCC in vitro.
From: Control of acute myeloid leukemia by a trifunctional NKp46-CD16a-NK cell engager targeting CD123
a, Comparison of the cytotoxicities of an anti-B7-H3 antibody (B7-H3-IgG1) and of an anti-B7-H3 NKCE molecule co-engaging NKp46 and CD16a (B7-H3-NKCE). CD64−negative A375 and LN229 cells, and CD64-positive THP-1 cells were used as the targets and purified resting NK cells as effectors. The data shown are representative of three independent experiments. b, Phenotype of the target cells used in a showing the expression of B7-H3, CD32a/b, CD64 and CD123 by flow cytometry.
