Fig. 3: Chem-map reveals an open chromatin binding preference for doxorubicin. | Nature Biotechnology

Fig. 3: Chem-map reveals an open chromatin binding preference for doxorubicin.

From: Chem-map profiles drug binding to chromatin in cells

Fig. 3

a, Chemical structure of biotinylated derivatives of doxorubicin. b, Microscopy analysis of U2OS cells visualizing nuclear enrichment of doxorubicin derivatives. Live U2OS cells were treated with doxorubicin and its derivatives for 6 h. Nuclei were stained with Hoechst 33342. BFB was used for visualization of nuclei (blue) and RFB was used for visualization of doxorubicin and its derivatives (red). Scale bar, 100 μm. Experiments were repeated independently three times. c, PCA analysis showing the distinct binding profiles of small molecules that have different protein and DNA targets in K562 cells. d, Venn diagram showing the overlap of doxorubicin binding sites with open chromatin mapped by ATAC-seq. e, Genome browser views of doxorubicin Chem-map binding sites (red) compared to a biotin control (gray) and ATAC-seq (black). BFB, blue-light filter cube; RFB, red-light filter cube.

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