Extended Data Fig. 9: USeqFISH validation in NHP tissue.
From: Spatial transcriptomics for profiling the tropism of viral vectors in tissues
a, Virally labeled cells, expressing mTurquoise2 (pseudo-colored), in the rhesus macaque brain and USeqFISH labeling with probes targeting the sequence of mTurquoise2. b, We validated our probe design for the rhesus macaque by applying USeqFISH with Pvalb probes and post hoc IHC with Pvalb antibodies to the same tissue. Localization of Pvalb mRNA signal in cells labeled with Pvalb antibodies (the same approach used for mouse probe validation in Supplementary Fig. 3) supports the versatility of our probe design in both rodents and NHPs.
