Extended Data Fig. 1: Further characterization of RCAHCR amplification.
From: Spatial transcriptomics for profiling the tropism of viral vectors in tissues
a, Representative images of Gad1 in mouse brain tissue detected by HCR, RCA for 2 hours (RCA 2 h), RCA overnight, and RCAHCR. The intensity of each image was adjusted for better visualization. b, Quantification of spot sizes automatically detected by Imaris in each condition (n = 74,292 (HCR), 10,744 (RCA 2 h), 11,608 (RCA overnight), 24,798 spots (RCAHCR); ****p < 0.0001, two-sided Mann-Whitney test). c, Representative images of Gad1 in mouse brain tissue detected by RCA 2 h, RCA overnight, and RCAHCR. The intensity range of all images was matched to show the different brightness of the signal. d, Cumulative distribution of RNA spot intensity of RCA 2 h, RCA overnight, and RCAHCR. Dashed lines indicate the mean of each distribution (****p < 0.0001, Kolmogorov-Smirnov test). e, The false-positive detection of RCAHCR in cell culture (left) and in tissue (right).
