Extended Data Fig. 7: CROP-seq with PIP-seq.
From: Microfluidics-free single-cell genomics with templated emulsification
(a) Gene expression for each sgRNA within an allelic series for all genes in the CRISPRi library. Each sgRNA is ordered from predicted high to low knockdown efficiency. Non-targeting sgRNA are denoted as “Null.” Data is from one CROP-seq experiment. Box plots indicate the median with the lower and upper hinges corresponding to the 25th and 75th percentiles. Raw data points are displayed with a slight jitter. (b) The relationship between gene expression and predicted knockdown of each gene. Expected changes in transcription across the allelic series were prominent in highly expressed genes. p-value represents the significance of the generalized additive model relating gRNA identity to knockdown efficiency for each gene. P-values for each model were directly plotted along with the average expression for each gene (using log1p of the normalized counts). The horizontal red line shows the significance level of p = 0.05.
