Extended Data Fig. 3: Quality control analysis of PIP-seq using healthy breast tissue.
From: Microfluidics-free single-cell genomics with templated emulsification
(a) Integration and clustering of 54,825 cells from 2 patients with 2 replicates per patient. (b) Coloring of UMAP by the number genes (nFeature RNA) for each cell. (c) The number of unique genes (nFeature RNA), transcripts (nCount RNA), percent mitochondrial reads, and percent ribosomal reads as a function of cluster. (d) Comparison between 10X Genomics’ and PIP-seq data after downsampling 2400 cells to 36,500 reads per cell. Box plots indicate the median with the lower and upper hinges corresponding to the 25th and 75th percentiles. (c,d) Each violin represents a combination of 4 individual samples (2 replicates from 2 patients).
