Extended Data Fig. 10: Single-cell epitope analysis of validation targets in colorectal cancer.
From: Single-cell mapping of combinatorial target antigens for CAR switches using logic gates
a, Clustering and cell-type mapping of the tumor and tumor-infiltrating normal cells based on the CITE-seq transcriptome of our pooled CRC samples. b, Single-cell maps, as in (a), overlaid with mRNA expression levels retrieved from the CITE-seq experiments. c, Single-cell maps, as in (b), overlaid with protein expression levels retrieved from the CITE-seq experiments. d, Distribution of protein expression levels broken down by annotated cell type. Log2 fold changes comparing CITE-seq ADT signals from targeting antibodies versus isotype controls were computed and are displayed for each protein in each cell type. e, Cell-by-cell protein expression patterns in the tumor and tumor-resident normal cells for CEACAM5 versus EPCAM, CEACAM5 versus CA4, CEACAM5 versus CPM, and CEACAM5 versus VSIG2. Cells enclosed in the marked zones indicate the expression of both proteins for the AND gating and only one protein (CEACAM5) for the NOT gating.
