Extended Data Fig. 9: Detection of transgene-free mutants with three pairs of primers based on the pH-ePPE-epegRNA, pH-ABE8e-spG and pH-ABE8e binary vector.
From: Tuning plant phenotypes by precise, graded downregulation of gene expression

Lanes with no bands generated by the three pairs of primers indicate transgene-free T1 mutants. M represents a DNA molecular weight ladder.