Fig. 4: Example of proteomics data corroborating occurrence of an alternative splicing (AS) event in APP.
From: Global detection of human variants and isoforms by deep proteome sequencing
The initial sequential order of exons undergoes transcription. Splicing processing follows, resulting in either 7–9 or 7–8–9 exon combinations. Since all mentioned exons are part of APP’s open reading frame, they have a theoretical possibility to be present and translated into a protein sequence. The multi-enzyme shotgun MS approach described here allows detection of peptides specific to each isoform. Two of 42 total spectra, corroborating these splicing events, are shown.
