Fig. 1: SPP for content analysis. | Nature Biotechnology

Fig. 1: SPP for content analysis.

From: High-throughput measurement of the content and properties of nano-sized bioparticles with single-particle profiler

Fig. 1

a, SPP setup. b, Fluorescence intensity traces and peak calling. c, Two-dimensional density map of fluorescence intensities for liposomes loaded with FAST DiO and Abberior Star Red DPPE at different ratios. d, Intensity distributions for each channel for the samples shown in c. e, Scheme for mRNA encapsulation of LNPs. f, Representative peaks for possible scenarios. g, LNP fraction with mRNA signals for different N/P ratios (colors represent the mean of one replicate). h, Ratiometric histogram of mRNA versus lipid dye intensity for single LNPs. i, Scheme for nanobody binding to VLPs. j, Representative peaks for possible scenarios. k, VLP fraction with nanobody signal for different nanobodies and different variants (colors represent the mean of one replicate). l, Ratiometric histogram of nanobody versus lipid dye intensity for single VLPs. All histograms are representative of three replicates.

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