Extended Data Fig. 6: HIV infection mediated CD4+ T cell depletion is not impacted by the presence of resident microbiota.
Flow cytometric analysis of a, human CD4+ T cells in the peripheral blood (PB) of viremic GF-BLT (red) and CV-BLT (black) mice at day 8 (GF, n = 4; CV n = 12), day 13 (GF, n = 4; CV n = 12), day 20 (GF, n = 4; CV n = 9), day 28 (GF, n = 4; CV n = 5) and day 35 (GF, n = 4; CV n = 4) post rectal HIV acquisition. Percent CD4+ of human T cells in the b, PB, bone marrow (BM), spleen (SPL), lymph nodes (LN), human thymic organoid (THY), liver (LIV), and lung (LNG) as well as the c, small intestine (S), cecum (C), and large intestine (L) intraepithelial layer (IEL) and lamina propria layer (LPL) of aviremic (filled bars) and viremic (open bars) GF-BLT mice. Aviremic GF-BLT mice, n = 9 (PB, BM, SPL, LN, THY, LIV), n = 8 (LNG, S IEL, C IEL, C LPL, L IEL, L LPL), or n = 5 (S LPL). Viremic GF-BLT mice, n = 4 (PB, BM, SPL, LN, THY, LIV, LNG) or n = 3 (S IEL, S LPL, C IEL, C LPL, L IEL, L LPL). Percent CD4+ of human T cells in the d, PB, BM, SPL, LN, THY, LIV, and LNG as well as the e, S IEL, S LPL, C IEL, C LPL, L IEL, and L LPL of aviremic (filled bars) and viremic (open bars) CV-BLT mice. Aviremic CV-BLT mice, n = 5 (PB, BM, SPL, LN, THY, LIV, LNG, S IEL, C IEL, C LPL, L IEL, L LPL), n = 4 (S LPL). Viremic CV-BLT mice, n = 8 (PB, BM, SPL, LN, LIV, LNG), n = 7 (S IEL, C IEL, C LPL, L IEL, L LPL), or n = 6 (THY, S LPL). SP, CD4+ single positive thymocyte. DP, CD4+CD8+ double positive thymocyte. b–e, Shown is the difference in percent CD4+ T cells between viremic and avirmic mice. Horizontal and vertical lines represent the mean and standard error mean respectively. a-e, Cell levels mice were compared with a two-sided Mann-Whitney test.
