Extended Data Fig. 10: Comparative analysis of prime editing and cDNA screening datasets of TP53 variants reveals pathogenic variants in the oligomerization domain.
From: High-throughput evaluation of genetic variants with prime editing sensor libraries
a, The difference in Z-scores between prime editing and cDNA screens (∆Z-score) for pegRNAs ≥ 30% editing, separated by p53 domain. Statistics shown for two-sided t-test with Bonferroni correction. OD vs. DBD (p = 3.255e-50), vs. PRR (p = 3.158e-17), vs. TAD (p = 2.085e-11). * = p-value ≤ 0.05, ** = p-value ≤ 0.01, *** = p-value ≤ 0.001, **** = p-value ≤ 0.0001, ns = not significant (p-value > 0.05). Boxes indicate the median and interquartile range (IQR) for each sample with whiskers extending 1.5 × IQR past the upper and lower quartiles. All z-scores were calculated from each pegRNA’s LFC, in turn calculated using MAGeCK from the median values across n = 3 biologically independent samples. b, Z-scores for variants in the cDNA (red) and prime editing (blue) screens, considering only the most efficient pegRNA for each variant with an editing efficiency ≥ 10%. c, Scatterplot of the cDNA ∆RFP % (day 10) and pegRNA ∆RFP % (day 14) for variants tested with competition assays. rp = Pearson correlation; rs = Spearman correlation.
