Extended Data Fig. 1: Generation of a TP53 prime editing sensor library with PEGG.
From: High-throughput evaluation of genetic variants with prime editing sensor libraries
a, Schematic of the PEGG pipeline. PEGG takes as input a list of mutations from the cBioPortal, ClinVar identifiers, or custom mutations sets, and produces user-defined pegRNA designs ranked by PEGG score, custom sensor oligos, and visualization tools. PEGG filters pegRNAs with polyT sequences as well as designs containing restriction sites used for cloning. With the library design feature, PEGG can also automate the aggregation of variants located in genes of interest, and automatically design a defined fraction of silent variant-generating pegRNAs. b, Breakdown of the TP53 variants input to PEGG for sensory library design. c, The fraction of input variants amenable to prime editing (that is, able to generate ≥ 1 pegRNA), separated by variant type. d, Histogram of the number of pegRNA designs per variant. e, Histogram of the MIT specificity score of the protospacers for the pegRNAs included in the library. The library was filtered to exclude pegRNAs containing a protospacer with an MIT specificity score less than 50.
