Extended Data Fig. 3: The TP53 prime editing sensor screen is highly reproducible with low indel rates.
From: High-throughput evaluation of genetic variants with prime editing sensor libraries
a, Pearson correlation in raw pegRNA counts among each replicate and time-point. Plasmid = plasmid library. b, Spearman correlation in sensor correct editing percentage among each replicate and time-point for pegRNAs with at least 10 sensor reads. c, Median indel percentage among active pegRNAs (≥1% editing) for each time-point and condition. Data are presented as mean values with a 95% confidence interval. d, Boxplot of indel frequency among active pegRNAs (≥1% editing) for each replicate and time-point. Lower quartile = 0% for all replicates (not visible). Boxes indicate the median and interquartile range (IQR) for each sample with whiskers extending 1.5 × IQR past the upper and lower quartiles. N = 3 biologically independent replicates in each condition shown in (c-d). e, Histogram of pegRNA editing efficiency in the Nutlin-treated Day 34 samples. f, Histogram of pegRNA editing efficiency of the most efficient pegRNA for each variant in the Nutlin-treated Day 34 samples. g, Comparative PDF of all pegRNAs and the most efficient pegRNA for each variant.
