Extended Data Fig. 2: Characterization of melanin production rates in differing melanin development buffer formulations. | Nature Biotechnology

Extended Data Fig. 2: Characterization of melanin production rates in differing melanin development buffer formulations.

From: Self-pigmenting textiles grown from cellulose-producing bacteria with engineered tyrosinase expression

Extended Data Fig. 2

a, Time course experiment of eumelanin accumulation at OD405 from K. rhaeticus tyr1 in a range of pH values. Phosphate buffered saline (PBS) set to 7.4 containing 0.5 g/L L-tyrosine and 10 μM CuSO4 is shown in gray. Points represent the mean while error bars represent the SD of 6 replicates. b, Average initial reaction rates of melanin accumulation from K. rhaeticus tyr1 in buffers of differing pH values. Reaction rates are derived from the gradient of OD405 over the first 140 minutes of the experiment. Colors match those in panel a. Bars represent the mean rate while error bars represent the SD of 6 replicates. c,d, Time course experiment of melanin accumulation and initial reaction rates of melanin accumulation from K. rhaeticus WT. Points and bars represent the mean while error bars represent SD of 6 technical replicates. e, Time course experiment of melanin accumulation at OD405 from K. rhaeticus tyr1 in differing PBS concentrations. A PBS concentration of 0x corresponds to ddH2O containing only melanin development substrates (L-tyrosine and CuSO4) while a PBS concentration of 1× corresponds to 137 mM NaCl, 2.7 mM KCL, 10 mM Na2HPO4 and 1.8 mM KH2PO4. Melanin development substrates are maintained at 0.5 g/L tyrosine and 10 μM CuSO4 for all PBS concentrations. Points represent the mean while error bars represent SD of 6 replicates. f, Average initial reaction rates of melanin accumulation from K. rhaeticus tyr1 in differing PBS concentrations. Reaction rates are derived from the gradient of OD405 over the first 140 minutes of the experiment. Error bars represent the SD of 6 replicates. g, Average initial reaction rates of melanin accumulation from K. rhaeticus tyr1 in buffers containing different metal (II) ions. Ions present in solution at 20 μM. Error bars represent the SD of 6 replicates. h, Average initial reaction rates of melanin accumulation from K. rhaeticus tyr1 in buffers containing different copper (II) concentration. Error bars represent the SD of 6 replicates.

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