Extended Data Fig. 7: CAFs promote an autoinflammatory program in CRC cells.
From: Patient-derived mini-colons enable long-term modeling of tumor–microenvironment complexity
a, Gene interaction network of the genes induced by CAFs and CAF-conditioned medium in CRC mini-colons. The zoomed-in area is indicated with a dashed rectangle. b, Quantitation of the invasive front reach in CRC mini-colons treated with the indicated molecules. *P = 0.0119, ***P < 0.0001 (two-way ANOVA and Sidak’s multiple comparisons test; n = 24 invasive fronts for each group). c, Signal intensity profile of MMP7 and CDH1 (E-cadherin) immunofluorescent stainings along IL1B-induced invasive fronts in CRC mini-colons. The tip of the invasive front is located at 100 μm. n = 3 for each staining. d,e, Quantitation of the invasive front reach (d) and epithelium thickness (e) in CRC mini-colons co-cultured with autologous CAFs and treated with the indicated compounds. **P = 0.0025; ***P = 0.0007 (e), < 0.0001 (d) (two-way ANOVA and Sidak’s multiple comparisons test; n = 24 invasive fronts (d) and 18 inter-crypt epithelium sections (e) for each group). f, Brightfield images of CRC mini-colons treated with the indicated compounds. Scale bar, 100 μm. In b-e, data represent mean ± SEM. In all panels, data refer to patient #MS.
